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lightcycler r 96  (Roche)


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    Structured Review

    Roche lightcycler r 96
    Lightcycler R 96, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lightcycler+r+96/LightCycler+480+System/pm41103024-111-25-31
    Average 99 stars, based on 6 article reviews
    lightcycler r 96 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A Diagnostic Survey of Aborted Equine Fetuses and Stillborn Premature Foals in Denmark.
    Article Snippet: The Master mixes containing the primers and the enzymes were automatically pipetted and mixed with the DNA/RNA by another Hamilton Microlab STARlet (Hamilton Germany GmbH) in a separate clean room. .. Positive and negative controls were included with every PCR, which was performed using a LightCycler R© 96 (Roche Diagnostics GmbH). ..

    Article Title: Elucidation of the hepatoprotective effect and mechanism of Melastoma dodecandrum Lour. based on network pharmacology and experimental validation
    Article Snippet: .. Genes was examined quantitatively using RT-qPCR by exposing cDNA to PCR amplification in a LightCycler R 96 (Roche, Basel, Switzerland). ..

    Quantitative RT-PCR:

    Article Title: Effect of Storage Temperature on Fruit Hardness and Anthocyanin Biosynthesis in Red Peeled Banana (Musa acuminata Hongmeiren)
    Article Snippet: The qRT-PCR mixture (20 mL in total volume) included 10 mL of 2× PerfectStart R Green qPCR SuperMix (TransGen Biotech, China), 0.4 mL of each primer (10 mM), 1 mL of cDNA, and 8.2 mL nuclease free water. .. Subsequently, the qRT-PCR was performed on a LightCycler R 96 real-time PCR instrument (Roche, Switzerland), initiated at 94 C for 30 s, followed by 40 cycles at 94 C for 5 s, 60 C for 30 s. Relative expression level was quantified using the 2 DDCt method and normalized to the endogenous control MaActin (Livak and Schmittgen 2001). ..

    Article Title: Elucidation of the hepatoprotective effect and mechanism of Melastoma dodecandrum Lour. based on network pharmacology and experimental validation
    Article Snippet: .. Genes was examined quantitatively using RT-qPCR by exposing cDNA to PCR amplification in a LightCycler R 96 (Roche, Basel, Switzerland). ..

    Article Title: Intranuclear paraspeckle-circular RNA TACC3 assembly forms RNA-DNA hybrids to facilitate MASH-related hepatocellular carcinoma growth in an m 6 A-dependent manner.
    Article Snippet: .. The RT-qPCR was carried out by a reaction mixture composed of the SYBR Green Premix Pro Taq HS qPCR Kit (#AG11701, Accurate Biology), using the LightCycler R© 96 Real-Time PCR System (Roche, Basel, Switzerland). .. The RT-qPCR was carried out by a reaction mixture composed of the SYBR Green Premix Pro Taq HS qPCR Kit (#AG11701, Accurate Biology), using the LightCycler R© 96 Real-Time PCR System (Roche, Basel, Switzerland).

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of Storage Temperature on Fruit Hardness and Anthocyanin Biosynthesis in Red Peeled Banana (Musa acuminata Hongmeiren)
    Article Snippet: The qRT-PCR mixture (20 mL in total volume) included 10 mL of 2× PerfectStart R Green qPCR SuperMix (TransGen Biotech, China), 0.4 mL of each primer (10 mM), 1 mL of cDNA, and 8.2 mL nuclease free water. .. Subsequently, the qRT-PCR was performed on a LightCycler R 96 real-time PCR instrument (Roche, Switzerland), initiated at 94 C for 30 s, followed by 40 cycles at 94 C for 5 s, 60 C for 30 s. Relative expression level was quantified using the 2 DDCt method and normalized to the endogenous control MaActin (Livak and Schmittgen 2001). ..

    Article Title: Inhibition of 15-PGDH by SW033291 ameliorates age-related heart failure in mice.
    Article Snippet: To remove any potential genomic DNA, an equal amount of total RNA was treated with gDNA Eraser reagent before being utilized for cDNA synthesis (Takara Bio, Shiga, Japan). .. Using a SYBR R Premix Ex TaqTM II kit (Takara Bio), qPCR amplification analysis was performed on a LightCycler R 96 (Roche, Basel, Switzerland). ..

    Article Title: Angiotensin receptor-neprilysin inhibitor improves coronary collateral perfusion.
    Article Snippet: .. The quantitative polymerase chain reaction (qPCR) based analysis was performed by using the LightCycler R© 96 Real-Time PCR Frontiers in Cardiovascular Medicine 05 frontiersin.org System (Roche). .. Each reaction system consists of 1 μl cDNA, 1 μl of forward/reverse primer each, 7 μl RNase/DNase-free water and 10 μl PowerTrack SYBR Green Master Mix (Thermo Fisher Scientific).

    Article Title: Intranuclear paraspeckle-circular RNA TACC3 assembly forms RNA-DNA hybrids to facilitate MASH-related hepatocellular carcinoma growth in an m 6 A-dependent manner.
    Article Snippet: .. The RT-qPCR was carried out by a reaction mixture composed of the SYBR Green Premix Pro Taq HS qPCR Kit (#AG11701, Accurate Biology), using the LightCycler R© 96 Real-Time PCR System (Roche, Basel, Switzerland). .. The RT-qPCR was carried out by a reaction mixture composed of the SYBR Green Premix Pro Taq HS qPCR Kit (#AG11701, Accurate Biology), using the LightCycler R© 96 Real-Time PCR System (Roche, Basel, Switzerland).

    Expressing:

    Article Title: Effect of Storage Temperature on Fruit Hardness and Anthocyanin Biosynthesis in Red Peeled Banana (Musa acuminata Hongmeiren)
    Article Snippet: The qRT-PCR mixture (20 mL in total volume) included 10 mL of 2× PerfectStart R Green qPCR SuperMix (TransGen Biotech, China), 0.4 mL of each primer (10 mM), 1 mL of cDNA, and 8.2 mL nuclease free water. .. Subsequently, the qRT-PCR was performed on a LightCycler R 96 real-time PCR instrument (Roche, Switzerland), initiated at 94 C for 30 s, followed by 40 cycles at 94 C for 5 s, 60 C for 30 s. Relative expression level was quantified using the 2 DDCt method and normalized to the endogenous control MaActin (Livak and Schmittgen 2001). ..

    Control:

    Article Title: Effect of Storage Temperature on Fruit Hardness and Anthocyanin Biosynthesis in Red Peeled Banana (Musa acuminata Hongmeiren)
    Article Snippet: The qRT-PCR mixture (20 mL in total volume) included 10 mL of 2× PerfectStart R Green qPCR SuperMix (TransGen Biotech, China), 0.4 mL of each primer (10 mM), 1 mL of cDNA, and 8.2 mL nuclease free water. .. Subsequently, the qRT-PCR was performed on a LightCycler R 96 real-time PCR instrument (Roche, Switzerland), initiated at 94 C for 30 s, followed by 40 cycles at 94 C for 5 s, 60 C for 30 s. Relative expression level was quantified using the 2 DDCt method and normalized to the endogenous control MaActin (Livak and Schmittgen 2001). ..

    Amplification:

    Article Title: Elucidation of the hepatoprotective effect and mechanism of Melastoma dodecandrum Lour. based on network pharmacology and experimental validation
    Article Snippet: .. Genes was examined quantitatively using RT-qPCR by exposing cDNA to PCR amplification in a LightCycler R 96 (Roche, Basel, Switzerland). ..

    Article Title: Inhibition of 15-PGDH by SW033291 ameliorates age-related heart failure in mice.
    Article Snippet: To remove any potential genomic DNA, an equal amount of total RNA was treated with gDNA Eraser reagent before being utilized for cDNA synthesis (Takara Bio, Shiga, Japan). .. Using a SYBR R Premix Ex TaqTM II kit (Takara Bio), qPCR amplification analysis was performed on a LightCycler R 96 (Roche, Basel, Switzerland). ..

    SYBR Green Assay:

    Article Title: Intranuclear paraspeckle-circular RNA TACC3 assembly forms RNA-DNA hybrids to facilitate MASH-related hepatocellular carcinoma growth in an m 6 A-dependent manner.
    Article Snippet: .. The RT-qPCR was carried out by a reaction mixture composed of the SYBR Green Premix Pro Taq HS qPCR Kit (#AG11701, Accurate Biology), using the LightCycler R© 96 Real-Time PCR System (Roche, Basel, Switzerland). .. The RT-qPCR was carried out by a reaction mixture composed of the SYBR Green Premix Pro Taq HS qPCR Kit (#AG11701, Accurate Biology), using the LightCycler R© 96 Real-Time PCR System (Roche, Basel, Switzerland).



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